Cucurbit yellow stunting disorder virus p25 is a suppressor of post-transcriptional gene silencing.
Identifieur interne : 000653 ( Main/Exploration ); précédent : 000652; suivant : 000654Cucurbit yellow stunting disorder virus p25 is a suppressor of post-transcriptional gene silencing.
Auteurs : Amr R A. Kataya [Grèce] ; Mohamed N S. Suliman ; Kriton Kalantidis ; Ioannis C. LivieratosSource :
- Virus research [ 1872-7492 ] ; 2009.
Descripteurs français
- KwdFr :
- ARN viral (génétique), ARN viral (métabolisme), Crinivirus (génétique), Crinivirus (métabolisme), Gènes viraux (MeSH), Interactions hôte-pathogène (MeSH), Interférence par ARN (MeSH), Maladies des plantes (génétique), Maladies des plantes (virologie), Petit ARN interférent (métabolisme), Protéines virales (génétique), Protéines virales (métabolisme), Protéines à fluorescence verte (MeSH), Tabac (génétique), Tabac (métabolisme), Tabac (virologie).
- MESH :
- génétique : ARN viral, Crinivirus, Maladies des plantes, Protéines virales, Tabac.
- métabolisme : ARN viral, Crinivirus, Petit ARN interférent, Protéines virales, Tabac.
- virologie : Maladies des plantes, Tabac.
- Gènes viraux, Interactions hôte-pathogène, Interférence par ARN, Protéines à fluorescence verte.
English descriptors
- KwdEn :
- Crinivirus (genetics), Crinivirus (metabolism), Genes, Viral (MeSH), Green Fluorescent Proteins (MeSH), Host-Pathogen Interactions (MeSH), Plant Diseases (genetics), Plant Diseases (virology), RNA Interference (MeSH), RNA, Small Interfering (metabolism), RNA, Viral (genetics), RNA, Viral (metabolism), Tobacco (genetics), Tobacco (metabolism), Tobacco (virology), Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , genetics : RNA, Viral, Viral Proteins.
- chemical , metabolism : RNA, Small Interfering, RNA, Viral, Viral Proteins.
- chemical : Green Fluorescent Proteins.
- genetics : Crinivirus, Plant Diseases, Tobacco.
- metabolism : Crinivirus, Tobacco.
- virology : Plant Diseases, Tobacco.
- Genes, Viral, Host-Pathogen Interactions, RNA Interference.
Abstract
Post-transcriptional gene silencing (PTGS) degrades RNA in a sequence-specific manner and is utilised by plants as a natural defence mechanism against virus invaders. Two members of the genus Crinivirus have been reported to encode suppressors and counter PTGS: Sweet potato chlorotic stunt virus p22 and Tomato chlorosis virus (ToCV) p22, coat protein and coat protein minor. Using an Agrobacterium-mediated transient assay on Nicotiana benthamiana wildtype and 16c plants, we screened four Cucurbit yellow stunting disorder virus (CYSDV) RNA 1-encoded proteins (papain-like protease, p25, p5.2 and p22) to determine which one possess PTGS suppressor activity. Amongst these proteins, only CYSDV p25 was able to suppress (double- and single-stranded) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by CYSDV p25 in both of these experiments had no apparent effect on the accumulation of the small interfering RNAs. The identification of CYSDV p25 adds to the list of suppressors encoded by crinivirus RNA 1 molecules, which are unrelated in terms of amino acid sequence homology suggesting distinct PTGS suppression mechanisms and possible roles in viral replication.
DOI: 10.1016/j.virusres.2009.06.010
PubMed: 19540278
Affiliations:
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Two members of the genus Crinivirus have been reported to encode suppressors and counter PTGS: Sweet potato chlorotic stunt virus p22 and Tomato chlorosis virus (ToCV) p22, coat protein and coat protein minor. Using an Agrobacterium-mediated transient assay on Nicotiana benthamiana wildtype and 16c plants, we screened four Cucurbit yellow stunting disorder virus (CYSDV) RNA 1-encoded proteins (papain-like protease, p25, p5.2 and p22) to determine which one possess PTGS suppressor activity. Amongst these proteins, only CYSDV p25 was able to suppress (double- and single-stranded) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by CYSDV p25 in both of these experiments had no apparent effect on the accumulation of the small interfering RNAs. The identification of CYSDV p25 adds to the list of suppressors encoded by crinivirus RNA 1 molecules, which are unrelated in terms of amino acid sequence homology suggesting distinct PTGS suppression mechanisms and possible roles in viral replication.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19540278</PMID><DateCompleted><Year>2009</Year><Month>11</Month><Day>17</Day></DateCompleted><DateRevised><Year>2009</Year><Month>09</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-7492</ISSN><JournalIssue CitedMedium="Internet"><Volume>145</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Virus research</Title><ISOAbbreviation>Virus Res</ISOAbbreviation></Journal><ArticleTitle>Cucurbit yellow stunting disorder virus p25 is a suppressor of post-transcriptional gene silencing.</ArticleTitle><Pagination><MedlinePgn>48-53</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.virusres.2009.06.010</ELocationID><Abstract><AbstractText>Post-transcriptional gene silencing (PTGS) degrades RNA in a sequence-specific manner and is utilised by plants as a natural defence mechanism against virus invaders. Two members of the genus Crinivirus have been reported to encode suppressors and counter PTGS: Sweet potato chlorotic stunt virus p22 and Tomato chlorosis virus (ToCV) p22, coat protein and coat protein minor. Using an Agrobacterium-mediated transient assay on Nicotiana benthamiana wildtype and 16c plants, we screened four Cucurbit yellow stunting disorder virus (CYSDV) RNA 1-encoded proteins (papain-like protease, p25, p5.2 and p22) to determine which one possess PTGS suppressor activity. Amongst these proteins, only CYSDV p25 was able to suppress (double- and single-stranded) RNA-induced silencing of the green fluorescent protein (GFP) mRNA. Restoration of GFP expression by CYSDV p25 in both of these experiments had no apparent effect on the accumulation of the small interfering RNAs. The identification of CYSDV p25 adds to the list of suppressors encoded by crinivirus RNA 1 molecules, which are unrelated in terms of amino acid sequence homology suggesting distinct PTGS suppression mechanisms and possible roles in viral replication.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kataya</LastName><ForeName>Amr R A</ForeName><Initials>AR</Initials><AffiliationInfo><Affiliation>Department of Sustainable Agriculture, Mediterranean Agronomic Institute of Chania, Alsylio Agrokepion, Crete, Greece.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suliman</LastName><ForeName>Mohamed N S</ForeName><Initials>MN</Initials></Author><Author ValidYN="Y"><LastName>Kalantidis</LastName><ForeName>Kriton</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Livieratos</LastName><ForeName>Ioannis C</ForeName><Initials>IC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>06</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Virus Res</MedlineTA><NlmUniqueID>8410979</NlmUniqueID><ISSNLinking>0168-1702</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D034741">RNA, Small Interfering</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D029383" MajorTopicYN="N">Crinivirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005814" MajorTopicYN="N">Genes, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="Y">RNA 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MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>02</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>05</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>06</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>6</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>6</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>11</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19540278</ArticleId><ArticleId IdType="pii">S0168-1702(09)00219-6</ArticleId><ArticleId IdType="doi">10.1016/j.virusres.2009.06.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Grèce</li></country></list><tree><noCountry><name sortKey="Kalantidis, Kriton" sort="Kalantidis, Kriton" uniqKey="Kalantidis K" first="Kriton" last="Kalantidis">Kriton Kalantidis</name><name sortKey="Livieratos, Ioannis C" sort="Livieratos, Ioannis C" uniqKey="Livieratos I" first="Ioannis C" last="Livieratos">Ioannis C. Livieratos</name><name sortKey="Suliman, Mohamed N S" sort="Suliman, Mohamed N S" uniqKey="Suliman M" first="Mohamed N S" last="Suliman">Mohamed N S. Suliman</name></noCountry><country name="Grèce"><noRegion><name sortKey="Kataya, Amr R A" sort="Kataya, Amr R A" uniqKey="Kataya A" first="Amr R A" last="Kataya">Amr R A. Kataya</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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